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RAPID MICROPROPAGATION OF
GRAPEVINE CV.
AGIORGITIKO THROUGH LATERAL BUD DEVELOPMENT
G. Banilas* and E. Korkas
Laboratory of Plant Biology &
Viticulture, Department of Oenology and Beverages
Technology, Technological & Educational Institute of Athens, Ag. Spyridona
Street,
12210 Aegaleo, Greece
* Author for correspondence (gban@teiath.gr)
Abstract
Micropropagation has been a well-established methodology for in vitro
regeneration of grapevine (Vitis vinifera L.), usually through shoot apical
meristem culture or adventitious bud formation. However, recent studies have
shown that micropropagated plants often show genetic discrepancy from the mother
plant, due to high levels of PGRs applied. Here we present an efficient protocol
for rapid in vitro regeneration of grapevine cv. Agiorgitiko genotypes through
nodal culture.
Development of a single main shoot was prominent on agar-solidified basal MS
medium without any PGR or supplemented with relatively low levels (e.g., up to
2.5 µM) of benzyladenine (BA). At higher BA levels axillary bud growth and shoot
multiplication were enhanced, but hyperhydricity was also apparent. Relatively
low concentrations of indole-3-butyric acid (IBA) promoted both the frequency of
shoots forming roots and the number of roots per shoot. Although the overall
yield is rather lower compared with shoot tip culture approaches, present
protocol may serve as an alternative for true-to-type regeneration of selected
clones of ‘Agiorgitiko’ and possibly of other grapevine cultivars.
Key words: cytokinins,
micropropagation, nodal culture, Vitis vinifera
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